Part:BBa_K211001:Design
RI7
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 454
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 428
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 834
Design Notes
Designed with PCR.
Primer design:
RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG
BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC
BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC
We would like to thank Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
Source
Rat olfactory protein, clone I7 (GenBank: M64386.1)
References
1.Radhika, V. et al . Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 , 325–330 (2007.)
2.Minic, J. et al. Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening. FEBS J. 272, 524–537 (2005).